An Optimized Protocol for First Strand cDNA Synthesis from Laser Capture Microdissected Tissue
نویسندگان
چکیده
منابع مشابه
Comparative genomic hybridization using DNA from laser capture microdissected tissue.
Comparative genomic hybridization (CGH) is a powerful screening technique that can identify regions of gain and loss within the whole genome in a single experiment. The combination of laser capture microdissection, whole-genome amplification, and CGH permits genomic screening with high specificity and sensitivity. This complement of techniques has enabled analysis of focal regions and subpopula...
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Laser-based microdissection technologies have been recently developed and applied to procure homogenous populations of tumor cells from paraffin-embedded and frozen tissue sections. When combined with whole-genomic amplification techniques, sufficient amounts of DNA can be generated from a small number of tumor cells procured by laser-based microdissection. Amplified DNA can then be used to per...
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Laser capture microdissection (LCM)-enabled region-specific tissue analyses are critical to better understand complex multicellular processes. However, current proteomics workflows entail several manual sample preparation steps and are challenged by the microscopic mass-limited samples generated by LCM, impacting measurement robustness, quantification and throughput. Here, we coupled LCM with a...
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ژورنال
عنوان ژورنال: Laboratory Investigation
سال: 2001
ISSN: 0023-6837,1530-0307
DOI: 10.1038/labinvest.3780329